Streptomyces Tyrosinase: Production and Practical Applications
نویسندگان
چکیده
Tyrosinases (monophenol, o-diphenol:oxygen oxidoreductase, EC (1.14.18.1) are copper-containg enzymes which catalyze the o-hydroxylation of monophenols and subsequent oxidation of o-diphenols to quinones. The enzymes are involved in the pigmentation and are important factors in wound healing and primary immune response. Tyrosinases are found in prokaryotic and eukaryotic microorganisms, in mammals, invertebrates and plants. Streptomyces is included in the Streptomycetaceae family and represents one of the most important genus of the Actinomycetales order due to its impressive number of species and their practical role. Members of this genus were deeply studied because of their capacity to produce antibiotics and enzymes of industrial importance as glucose isomerase, protease, amylase, xylanase, while their capacity to produce tyrosinase was studied in a lesser extent.
منابع مشابه
Tyrosinase Inheritance in Streptomyces Scabies. Ii. Induction of Tyrosinase Deficiency by Acridine Dyes.
Gregory, Kenneth F. (Ontario Agricultural College, Guelph, Ontario, Canada), and Jay C. C. Huang. Tyrosinase inheritance in Streptomyces scabies. II. Induction of tyrosinase deficiency by acridine dyes. J. Bacteriol. 87:1287-1294. 1964.-Growth in minimal medium containing 1 mug of acriflavine per ml resulted in a large increase (up to 62%) in the frequency of tyrosinase-deficient (tye(-)) mutan...
متن کاملDevelopment of bioconjugate from Streptomyces tyrosinase and gold nanoparticles for rapid detection of phenol constituents.
Most of the phenol compounds are toxic and have been considered as hazardous pollutants. Several physicochemical and biological methods are available to detect and monitor the phenol pollutants in water and soil. In the present study, phenol constituents of winery, paper and plastic industrial effluents were successfully detected employing tyrosinase-gold nanoparticles bioconjugate. The synthes...
متن کاملCloning and expression of the tyrosinase gene from Streptomyces antibioticus in Streptomyces lividans.
In two separate studies a BclI-generated DNA fragment coding for the enzyme tyrosinase, responsible for melanin synthesis, was cloned from Streptomyces antibioticus DNA into two SLP1.2-based plasmid vectors (pIJ37 and pIJ41) to generate the hybrid plasmids, designated pIJ700 and pIJ701, using S. lividans 66 as the host. The fragment (1.55 kb) was subcloned into the multicopy plasmid pIJ350 (whi...
متن کاملCloning and identification of a novel tyrosinase and its overexpression in Streptomyces kathirae SC-1 for enhancing melanin production.
A 30-kDa novel tyrosinase was purified to homogeneity. The Km for L-Dopa and L-tyrosine were determined as 0.42 and 0.25 mM. The 1231 bp (base pair) melC gene and its 167 bp promoter Pskmel were obtained by thermal asymmetric interlaced polymerase chain reaction based on the amino acids fragment obtained from MS results of the purified enzyme. The protein sequence of tyrosinase shows maximum id...
متن کاملTemperature-sensitive mutants of the Streptomyces plasmid pIJ702.
DNA from the Streptomyces plasmid pIJ702 was mutagenized in vitro using hydroxylamine and transformed into Streptomyces lividans. One plasmid with temperature-sensitive replication (pMT660) and one plasmid with a temperature-sensitive tyrosinase (pMT661) were isolated. The plasmid pMT661 contains a novel PstI restriction endonuclease site within the tyrosinase gene.
متن کامل